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Steps in the pathway of the thermal unfolding of ribonuclecase A
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8 shows results from our quantitative analysis of cavity statistics for the EV-limit ensembles of proteins
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Structural interpretation of the nanosecond-timescale internal motionThe dimeric CaM:SEF2-1mp complex can in principle show internal modes of motion involving either the individual N- and C- terminal domains and/or the CaM monomers as a whole
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4), the compressibility factor being ≤1 for mass fractions up to ~40% w/w
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0 the virial coefficient remained negative as also found by Schaink and Smit (40) using membrane osmometry (mixed BLG A and B) at pH 5
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We envision the use of these probes and other probes with similar properties and the application of some of the techniques described within to serve as useful tools for future scientific studies, diagnosis, and high throughput screening of misfolding inhibitors
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The A-state is of molten globule type and shows a dramatic increase in β-sheet content evident from a spectral peak at 213 nm (Fig
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DISCUSSIONIn this study, we have applied high pressure techniques to determine the volume change associated with PLCβ-Gβγ interactions on membrane surfaces and in buffer
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As an illustration, conformational entropy buildup curves as a function of block size with error bars derived from the block averaging procedure are presented for gp120 atom sets in Fig
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