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Fluorescence studies of the conformational dynamics of parvalbumin in solution: lifetime and rotational motions of the single tryptophan residue
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Chemical modification of two tryptophan residues abolishes the catalytic activity of aminoacylase
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As a representative for the first class, BLA shows a λ^sub max^-value for the native state of 333 nm, which increases up to 339 nm upon thermal unfolding
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Effect of process conditions on recovery of protein activity after freezing and freeze-drying
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NMR spectroscopyIf nothing else is mentioned, the NMR measurements were performed at 308 K
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DCVJ was excited at 470 nm and ThT at 440 nm
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The approach to generate these species in solution opens up the possibility to probe the process by a wide number of biochemical and biophysical techniques to provide insight into the mechanism of protein misfolding and misassembly
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CPM-labeled proteins were excited at 385 nm and scanned from 420 to 540 nm
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On the other hand, the EVA 19% dissolved at 70°C when held for 2 hours, and particles with average sizes of 24 µm formed
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To better understand the basis for the observed broadening and tailing, we carried out numerical simulations using a simple model of the single-molecule FRET data
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