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The native ACYI contained Class I (~60%) and II (~40%) fluorophores, but did not contain Classes A, S, or III (Fig black gambling jack rule .
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Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^ black gambling jack rule .
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RT^sup n^^sub 1^ only decreases below 1 when the internal motion is slower than 200 ps or when an additional internal motion with a timescale slower than 200 ps is present black gambling jack rule .
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