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Since gSBA and rSBA both exist as tetramers with identical ligand binding specificities and macromolecular properties, the change of the mode of unfolding from a two-state process to a three-state process and the decrease of T^sub m^ by 26°C for the thermal unfolding of the protein of the kilter implicate the covalenlly linked glycans for the stability of gSBA
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are in general agreement with each other and reinforce Tanford's hypothesis (3) that highly denatured proteins behave like chains in a good solvent
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The values we use for σ^sub ij^ and ε^sub ij^ have been published previously (48)
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If the EV interactions, shown in Eq
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org--California Dairy Research Foundation site on bioactives and probioticsReferences:(1) Baer DJ, et al
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2-4 were carried out by using the general curve-lit option of Kaleidagraph (Abelbeck software) working on a PC computer
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In conclusion, the results from both quenchers suggest that at acidic pH, the aromatic residues became accessible to the solvent, although they were not as exposed as when EI^sup sc^ was fully unfolded (7 M GdmHCl)
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Experiments were carried out at pH 4, 7, and 10
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J Immunol 2002; 169:3978-3986(25) Fadok VA, Bratton DL, Rose DM, et al
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However, this same aspect leads to a lower affinity and specificity than for (cooperative) rigid binding (with all target sites simultaneously and rigidly interacting)
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4 B), thus one exponent adequately fits the data at 100°C
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Given the height scaling used in these measurements, the elongated species are not loosely interacting groups of single particles, but, instead, unified entities
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Hydration and structure-the two sides of the insulin aggregation process
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5 and 6, and several corresponding pairs of rate constants (data not shown), and find these to be poorer descriptions of the experimental data as well
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A 100 µl sample of Hoechst (1 µg/ml) was added to each of the wells
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Effect of transforming growth factor-[beta] on proteoglycan synthesis by chondrocytes in relation to differentiation stage and the presence of pericellular matrix
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