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In contrast, the recombinant protein unfolds via an intermediate
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the high mannose GlcNAc^sub 2^Man^sub 9^ glycan chain was attached with Asn-75 (Nδ2) of the protein (Protein Data Bank code 2SBA) using MOE (Molecular Operating Environment) software, and energy was minimized using a force field of AMBER99
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Having stressed that the data of Fig
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The interaction is clearly different from the well-known categories of specific binding by lock-and-key or induced-fit binding, in which the complex in the bound state becomes essentially locked in one conformation
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At this pH the protein has a net negative charge, the dimer carrying a charge of -17e (36), where e is the protonic charge; and so the apparent specific volume would be expected to have a contribution from net repulsive electric double layer interactions in addition to the excluded volume of the hydrated protein itself
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The magnitude of incremental contributions to Φ from the gutter potentials is restricted to these low values only in very simple topologies such as the three-helix bundle
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A total of seven P
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These data are overlaid on the shift plots in Fig
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