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. Classical properties of the MG state include increased molecular volume caused by an increase in internal water content (Kuwajima, 1989), preservation of nativelike secondary structure (Nozaka et al
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. The order of events could be characterized as the intensity decrease of the band at 1629 cm^sup -1^ > the intensity increase of the band at 1617 cm^sup -1^ > the intensity decrease of the band at 1649 and 1658 cm^sup -1^
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. For all three fluxes shown in Fig
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. The viscous behavior of globular protein solutions will therefore be strongly influenced by the characteristics of this hydration layer
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. In this case the "funnel" only appears in highly projected coordinates that follow successive reductions in the dimension of the search space
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. In that sense, the role of nonnative interactions in determining the type and rate of folding pathways is not a new idea (Zhou and Karplus, 1999)
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. Furthermore, fluorescence spectral shift data suggested that the unfolding of GR was complete at 3 M GdnHCl (Fig
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. No significant difference was observed when the scanning rate was increased from 0
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. Of particular interest is the value of R^sub o^, which is related to the persistence length and also provides a measure of the goodness of the solvent because R^sup 3^^sub o^ quantifies the average volume per residue set aside by the chain for interactions with the surrounding solvent (38,41)
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