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. We then "flip a two-sided coin" to decide if the trial move is going to be a backbone or side-chain move
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. Of course, what we refer to as cavities will actually be filled by solvent and cosolule molecules under denaturing conditions
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. CONCLUSIONS We have used an atomistic EV model, developed in previous work, to show that it is computationally tractable to generate accurate conformational ensembles for proteins in the EV limit
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. J Clin Invest 2002; 109:41-50(37) Odaka C, Mizuochi T, Yang J, et al
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. It should be noted, however, that B-factors from x-ray crystallography, in general, include contributions from static disorder, and care must be taken in comparing them to thermal atomic fluctuations (14)
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. lib and b^sup /^) than for 255°C (Fig
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. 5 in the presence of 80% glycerol clearly shows pH-independent fluorescence (Fig
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. Therefore, studies using pressure studies using Gα^sub s^ were limited to a maximum of 1 kbar in pressure
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. The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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. After 48-h incubation, the disk reconstitution appears incomplete and a sharp lipid transition of reduced amplitude is detected (Fig
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. The existing morphological studies on insulin aggregation stress the hierarchical intertwisting of protofilaments (Jimenez et al
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. 1 q) and no more oligomers are detected
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. The overlay with the experimental data (burs) shows that the simulations describe the data fairly well, given the simplicity of the model
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- Fourth Presbyterian Church in Chicago has explored a similar venture, though approval by the city is uncertain
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. In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998)
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