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The conformation changes cooperatively from native state to random coil between pH = 5 and 3 bankruptcy home loan new .
This limit is of interest in light of data that suggest the presence of residual structure even under strongly denaturing conditions bankruptcy home loan new .
As noted by Chan and Dill in their influential review (37), theories drawn from the polymer physics literature (38-44) are well-suited to describe heterogeneous conformational ensembles such as those of denatured slates bankruptcy home loan new .
FoId-X was also used to evaluate the interaction energy in the dimer formed by both C-terminal domains by calculating the difference in stability energy between the dimer and the isolated domains bankruptcy home loan new .
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Aggregates are expected to have rotational correlation times considerably longer than the decay time of the used dyes (e bankruptcy home loan new .
Transthyretin aggregation under partially denaturing conditions is a downhill polymerization bankruptcy home loan new .
In the basal state, Gα(GDP) is strongly bound to Gβγ-subunits, but GTP-bound Gα has a much weaker affinity (e bankruptcy home loan new .
In a study that focused on the interaction between PLCβ^sub 2^- and Gβγ-subunits, we found, not surprisingly, that the measured steady-state dissociation constant for the two proteins was equal to the ratio of the on- and off-rates (Runnels and Scarlata, 1998) bankruptcy home loan new .
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Large enthalpic (-259 ± 13 kJ µmol^sup -1^) and entropic changes (220 ± 13 kJ mol^sup -1^ at 310 K) upon binding were observed experimentally (Kwong et al bankruptcy home loan new .
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