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. No significant difference in the circular dichroism spectrum was seen in either the far UV (190-230 nm) or the near UV (260-300 nm) before and after the neutron scattering measurement
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. The same figure also shows the S^sup 2^^sub f^ contours when an additional nanosecond-timescale internal motion is present with time constant τ^sub is^ = 2 ns and order parameter S^sup 2^^sub s^ = 0
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. The Φ angles, recalculated with the smaller anisotropy, were essentially within the error margins of the earlier estimates
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. We thank Janny Hof for critical reading of the manuscript
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. At subsaturation levels of membranes, Gβγ dissociates above 1000 atm, which may be due to the phase transition, but at higher lipid concentrations, CPM-Gβγ membrane association is stable throughout the pressure range (Fig
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. This work was supported by the National Institutes of Health GM53132
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