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. In his new position, Milone will be responsible for compliance with federal and state regulatory requirements, litigation matters and company contracts
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. Similar results were obtained for all other two-state protein sequences shown in Table 1
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. The number and position of bands obtained from the deconvoluted (with a Lorentzian bandwidth of 18 cm^sup -1^ and a resolution enhancement factor of 2) and the Fourier derivative (with a power of 3 and a breakpoint of 0
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. To reduce the variability of fluorescence intensity in frozen samples, due to the poor homogeneity of the medium, the sample was rotated (3 Hz) during the measurement and the signal intensity was integrated for 10 s
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. The mouse major urinary protein:pheromone interaction (48) and the topoisomerase I domain interaction with single-stranded DNA (49) have, or suggest to have, the aspect of multiple-binding alternatives in the bound state
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. Furthermore, PrP^sup SC^ particles share many of the features of the toxic small prefibrillar species found in amyloid diseases
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. EXPERIMENTAL PROCEDURESProtein purificationTTR was expressed in BL21/DE3 cells transformed with the pmmHa plasmid
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. The Gβγ-subunits used in this study are geranylgeranylated, whereas Gα^sub s^-subunits are dually palmitoyated (Kleuss and Krause, 2003: Wedegaertner et al
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. Partially folded intermediates in insulin fibrillation
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. Role of asparagine/ glutamine repeat and nonrepeat regions of the prion domains
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. Homogeneous nucleation takes place at or near the surface of the globules, and growth proceeds along the globule walls, giving rise to smooth particles
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. The subsequent procedure was performed as described above
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. Ornithological Monographs 20:i-vi, 1-95
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. Conservation Studies on Raptors
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. During the in vitro monolayer expansion, the cells typically dedifferentiate to a fibroblastic morphology and phenotype
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. 16 Re-expression of a differentiated phenotype is enhanced by the presence of defined growth factors, such as TGF-[beta], insulin or IGF-I
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. Longitudinal studies are needed to address this issue
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. It is interesting to compare the efficiencies obtained for iodide quenching of the fluorescence of GR and of free Trp in aqueous solution
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. The proteins were redissolved in D^sub 2^O and centrifuged at 6000 × g for 10 min before use with a final pD of 8
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. Although a quantitative relation between aggregation and reduction of the unfolding induced red shifts cannot be given, our data indicate that protein aggregation contributes significantly to smaller magnitudes of red shifts as observed during thermal unfolding
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. At small strains, γG' over the frequency range examined of 0
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. Indeed, compaction potential appears to be inversely related to the binding activity of monocations
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. The second DSC peak is centered at 84°C, which is similar to the apparent T^sub m^ determined from the peak position in the first derivative of the CD melting data, dΘ^sub 222^(T)/dT (Fig
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. In the hypergutter picture, the nine-dimensional search space of these critical regions separately is reduced to three sequential three-dimensional searches by the long lifetimes of the native regions when the remainder of the protein is disordered
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. Ann Emerg Med 1995; 26: 187-94
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