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. Assuming an average lifetime of 3 ns for Trp in aqueous solution (Ferreira, 1989), the resulting value of K^sub SV^ for iodide quenching of Trp is 19
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. Each data point was an average of four accumulations
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. A 1-cm-pathlength quart/, cell (Hellma, Muellheim/Baden, Germany) was used
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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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. 1 was examined more extensively, resulting in the nonequilibrium phase diagram shown in Fig
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. 33 K/min (80 K/h) yields similar CD melting curves (not shown), with the apparent T^sub m^ = 84°C identical to that of intact disks
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. For example, curvature in the "Chevron plot" that results from a plot of log k^sub f^ with [denaturant] is taken to indicate a change of transition state (and by implication the existence of an intermediate)
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. THERMAL ANALYSISWe determined the crystalline melting temperatures T^sub m^, of the IPP, EVA 9%, and EVA 19% polymers by DSC as 165, 83, and 97°C, respectively
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. It has been proposed that the catalytic domain contains a β-sheet sandwiched between α-helices and a second β-sheet located on the surface, whereas the dimerization domain consists of a β-sheet flanked on one side by two α-helices (17)
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