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. One month later, the import bill cleared the House, but was later killed in the Senate
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. According to the neutral-mutation theory, however, if a genetic mutation leads to blindness of an eyed fish living in a cave, the fish will have no advantage or disadvantage over other eyed fish in the cave
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. The nonglycosylated form features a lower stability when compared to the glycosylated form
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. Similar results have been observed in 1), the enzyme malate synthase G (54), a close structural analog of pyruvate dikinase and EIC (11), where binding to pyruvate does not cause large structural changes (55); and 2), the pyruvate dikinase from maize, where only small changes are observed in selected regions upon binding of PEP (56)
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. The addition of PEP alone slightly destabilized the protein (Table 3), suggesting that the conformational changes monitored by trypsin digestion and quenching experiments did not result in large thermodynamic variations
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. Studies on the molecular weight and association of Enzyme I
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. Curr Biol 2004; 14:2208-2216(34) Fadok VA, Bratton DL, Guthrie L, et al
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. Temperature scans were performed using a constanttemperature cuvette holder connected to an external constant temperature circulator/bath (F25, Julabo, Seelbach, Germany; using a special Julabo bath fluid H10S)
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. On the other hand, WS flux shows the best performance for both solders with Ni substrate
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. For this reason, the IMC layer thickness was found from Fig
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. TTR is also associated with the sporadic disease senile systemic amyloidosis (Westermark et al
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. Previous work by us and others have employed protein engineering and cysteine labeling to attach specific probes to proteins to record conformational changes (Hammarström et al
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. The A-state is of molten globule type and shows a dramatic increase in β-sheet content evident from a spectral peak at 213 nm (Fig
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. Mapping the folding intermediate of human carbonic anhydrase II
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