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. The optimal temperature for ACYI activity is ~60°C (25,26), which indicates that it can be regarded as a thermostable enzyme
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. The data show that S [asymptotically =] 0
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. a sigmoidai curve with a pK^sub a^ of 4
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. However, due to the close proximity of all involved components, a new CaM:SEF2-1mp interaction is rapidly reformed, which in turn forces the CaM dimer to close again
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. The observation of 30% helical structure in 5-20 µg/ml apoA-2 in the absence of significant self-association, and the unfolding of this structure upon cooling (Fig
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. The Θ^sub 222^(t) data show a rapid signal loss at any Gdm HCl concentration, indicating fast unfolding within the dead-time of the experiment (30 s), which precludes its quantitative analysis; the amplitude of this rapid phase increases with increasing denaturant concentration (Fig
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. This work was supported by grants from the National Institutes of Health to D
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. The first peak is centered at 28°C and reflects DMPC phase transition in intact disks that is not detected by CD (Fig
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