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. Ensembles are characterized by large correlated fluctuations
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. Intestinal fatty acid binding protein: The folding mechanism as determined by NMR studies
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. lowest line) could be titled to a two-state mechanism, with T^sub m^ = 332 K (Table 3)
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. Adaptive ImmunityThe strongest evidence linking efferocytosis with the regulation of adaptive immunity is the association of human and murine autoimmune diseases with the impaired removal of apoptotic cells
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. In the same study we could also conclude that SEF2-1mp lacks any well-defined secondary structure when interacting with CaM, an observation very unusual for CaM-bound peptides
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. Aggregation occurs at the protein concentrations at which phase separation occurs (The observed phase behavior is consistent with literature measurements of both the second virial coefficient (39,40) and the solid-liquid coexistence line (41)
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. Protein aggregation and amyloid formation in vivo and in vitro are very complex processes which require sophisticated techniques for appropriate understanding and for future development of diagnostic tools and assays for high throughput drug screening
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. In this process too, there is strong evidence that the nonspecific interactions are themselves subtly coded to further speed the search for the binding target
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. , 1995), using a dielectric constant of 54
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. children and adults who were taking the medication
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. The other reagents used in this study were of the highest purity available
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. The inclusion bodies thus obtained were precipitated using 65% ice-cold acetone
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. 12 support the conclusion that EV limit ensembles are topologically equivalent
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. The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig
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. Localization errors in our experiments can hence be considered to be confined within each compartment
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. Heat capacities and a snapshot of the energy landscape in protein GB1 from the pre-denaturation temperature dependence of backbone NH nanosecond fluctuations
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.Here, p^sub A^ and p^sub B^ are the populations of the states A and B, Δ^sub ex^ = Ω^sub A^ - Ω^sub B^ is the chemical shift difference between the two states, k^sub ex^ = k^sub A[arrow right]B^ / p^sub B^ = k^sub B[arrow right]A^ / p^sub A^ is the rate constant for the exchange process, δ is the delay of the δ-180°-δ CPMG block and ω^sub I^ is the frequency of nucleus I
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. The +4°C shift, the reduced height, and the increased width (decreased cooperativity) of this peak as compared to the sharp transition in DMPC vesicles at 24°C (Chapman, 1975) are characteristic of lipoprotein disks that have smaller co-operative lipid domains than vesicles
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. The majority of mature amyloids are unbranched fibrils (Rochet and Lansbury, 2000) consisting of several, often intertwined protofilaments (which may form an internal waterfilled canal-Perutz et al
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. In the context of the three-helix protein, the necessary non-specific interactions are those that keep the first and third helices in contact
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. Mean ([+ or -] SE), range and number ofobservations are shown in the table
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. Canadian Journal of Zoology 60:1282-1288
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. We could not reject these hypotheses at the ,5 percent level (Table l)
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