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Nuclear magnetic relaxation studies of the role of the metal ion in Mn^sup 2+^-substituted aminoacylase I
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. For small x, the discrepancy is even more pronounced
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. 1 M^sup -1^) similar to those measured in isolated EI^sup SC^ (Table 1), and smaller when PEP (1
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. The triglyceride peaks were better resolved, and small additional peaks-creatine, choline, and phosphatidylcholine at 2
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. 5°C), mean-square displacements of native HuBChE were larger than those of its inhibited counterpart, which denatures only at 347
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. 5a-c for Ni substrate
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. Bound to aggregated TTR, the fluorescence lifetime of both components have increased and the relative amplitude of the slower component has increased (Table 3), well in accordance with the massive increase in quantum yield compared to when bound to native TTR (Fig
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. The body was elongate and relative body depth remained fairly constant throughout development
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. RESULTSConfigurational entropy of gp120 and CD4The configurational entropy of various sets of atoms of the free gp120 and CD4 molecules and their complex are shown in Figs
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. Postfertilization ovaries were very fragile and tended to rupture easily and release embryos under the slightest pressure
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. i] = subsample count i, where i=1 to 3; and SS[W
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. ) 7Faculty of Agriculture,Early 6 Jun '96Kyushu University(Fukuoka, Fukuoka Pref
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. Standard histology confirmed that cells cultured in DMEM/ITS+/TGF-[beta]1 generated larger pellets than those maintained in DMEM/ 10%FCS (Figs 2 to 4)
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. The "molten globule" state is involved in the translocation of proteins across membranes? FEBS Lett
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. with a T^sub m^ = 330 K (23)
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. Lower protein concentrations and smaller heating rates lead to larger red shifts upon thermal unfolding, indicating that under these conditions the protein aggregation is less pronounced
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. A possible explanation for this large red shift during thermal unfolding, which is almost as large as those obtained with GndHCl-induced unfolding (see Table 2), may be given by the fact that the absent aggregation gives rise to only a weak burial of tryptophan residues
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. The fluorescence intensity profiles of BSA, shown in Fig
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. Intracellular molecules have smaller diffusion coefficients than extracellular molecules because intracellular and compartmental boundaries restrict diffusion
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. Information on the dynamics of protein hydration may be obtained from ^sup 17^O magnetic relaxation dispersion measurements, which indicate that water molecules at protein surfaces are highly mobile (66,67)
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. Such prefibrillar oligomers could also serve as a common intermediate between the amyloidoses and other misfolding diseases, including prion diseases, Parkinson's disease, and amytrophic lateral sclerosis
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. The large number of these, of order N times greater than the number of native interactions, make them a likely candidate for information storage, as well as their natural propensity for kinetic control
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. Unlike other structural parameters such as the atom-positional root mean-square deviations (RMSD) of the trajectory structures from the starting structure, which seem to reach equilibrium after 2 ns for CD4 and 4 ns for gp120 (Fig
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. Pyruvate (1 mM), dexamethasone (10^sup -7^ M) and 10 ng/ml TGF-[beta]1 (recombinant human, R&D Systems, Abingdon, UK) were also added
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