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Fully unfolded monomers were obtained at higher concentrations of GdnHCl
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1 B), resulted in the full exposure of one SH group per subunit
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Although phase diagrams have usually been used in the analysis of Trp fluorescence data in previous publications, the theoretical analysis of the phase diagram is also expected to be true for IR data
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The high response of tetrameric proteins may suggest a particular destabilization of the native structure associated to the oligomeric state, a perturbation probably related to dissociation of the subunits and exposure of previously buried hydrophobic regions
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5) are identical for the folded native and soman-inhibited enzymes
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Upon peptide binding, the flexibility of side chains located in the binding sites is redistributed
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Heteronuclear (H-1,C-13,N-15) NMR assignments and secondary structure of the basic rcgion-helix-loop-helix domain of E47
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0 M NaCl at pH 5
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This work was supported through the core strategic grant of the United Kingdom Biotechnology and Biological Sciences Research Council
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Perhaps the most remarkable example is the determination by fast kinetic experiments that β -lactoglobulin employs a transient helical motif that is entirely nonnative (Park et al
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1 54EH-04/9865[degrees]13'Pseudopercis1492
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