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. But Jeffery's research showed that, even after tens of thousands of years of evolution in caves, most Astyanax genes that play a role in eye formation remain perfectly functional
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. the accessibility of atoms and residues, the backbone dihedral angles, and the hydrogen bond and electrostatic networks of the protein
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. Trypsin digestion experiments The digestion patterns in the presence of Mg^sup 2+^ showed the same behavior as in the absence of any effector: most of the protein was rapidly digested within the time of the experiments (open and solid squares)
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. We gratefully thank María T
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. Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli
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. The impact of aggregation on fluorescence emission spectra To investigate how fluorescence emission spectra are affected by aggregation we measured the aggregation directly by the use of elastic light scattering and we analyzed unfolding transitions under different conditions which lead to a different extent of protein aggregation
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. The spectrum of the slice through the nucleus hence contained cytoplasmic signal
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. Using CPM-PLCβ as a readout of possible conformational changes corresponding to Gβγ activation, we monitored CPM-PLCβ fluorescence under pressure and compared it directly to using CPM-PLCβ with supersaturating levels of Gβγ-subunits
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. The high-temperature transition in apoA-2: DMPC disks (solid circles, Fig
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. According to the definitions mentioned above, such folding intermediates of GR can possibly be described as MG states
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. The mean residue ellipticity of the recombinant protein was similar to that of the wild-type, suggesting that there are no major structural changes between the native glycosylated and recombinant nonglycosylated proteins (Fig
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