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. The original IR or fluorescence data were normalised by the corresponding intensity of the spectra recorded al 30°C
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. coelicolor were obtained by homology modeling
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. In contrast, the backbone within the domains is fairly rigid, both for target-free (3) and target-bound CaM (10)
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. The microscope was coupled to an AS-12 E-scanner (13-µm) and an Extender Electronics Module EX-II (Digital Instruments, Santa Barbara, CA), which allows acquisition of phase images
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. Interestingly, the clusters are not spherical, and pearl-like arrangements and elongated forms appear to dominate (examples are marked in Fig
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. As an example of the type of fine-structure predicted, the model contains a natural explanation of the curvature seen in plots of the denaturant dependence of folding rates
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. More detailed predictions of nonnative contributions to Φ for the family of bacterial immunity proteins and their mutants are in accord with very recent experiments
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. In addition, the existence of a partially denatured predissociated state has been proposed in the unfolding of the dimeric DNA-binding protein Arc represser (Peng et al
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. At first glance, these results are surprising since the highly denatured state is the starting point for in vitro folding reactions and yet no consideration of the denatured-state topology is required to account for the folding rates
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. The corresponding conformational entropy differences with their respective standard error estimates from the block averaging analysis are summarized in Table 2
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.Single-molecule methods represent an excellent tool to decipher the complexity of RNA folding processes, which generally proceed via intermediates and multiple pathways
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