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This indicates that dissociation of the GR dimer takes place in a narrow range of GdnHCl concentrations between 2 arizona home loan purchase .
The IR difference absorption spectra were calculated by subtracting the spectra at given temperatures from the spectrum recorded at 30°C (Fig arizona home loan purchase .
At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range arizona home loan purchase .
Conventional NMR experiments based on the nuclear Overhauscr effect are incapable of resolving contacts that go beyond 5-7 Å arizona home loan purchase .
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The high response of tetrameric proteins may suggest a particular destabilization of the native structure associated to the oligomeric state, a perturbation probably related to dissociation of the subunits and exposure of previously buried hydrophobic regions arizona home loan purchase .
Using a high magnetic field and a home-built microscopy probe with large gradient field strengths, we achieved voxel sizes of (180 µm)^sup 3^ arizona home loan purchase .
Lipid suppression has been especially important for selecting regions for localized spectroscopy, since at this high magnetic field strength, the lipid image of the lipid-rich oocyte significantly affects the water image, thus hampering correct voxel positioning arizona home loan purchase .
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1 B), and from the apparent melting temperatures determined by CD, light scattering, and DSC, T^sub m,apoA-1^ > T^sub m,apoA-2^ > T^sub m,apoC-1^ (Fig arizona home loan purchase .
The large-amplitude relocalization of the V3 loop of gp120 upon binding is, however, free from any substantial entropic cost arizona home loan purchase .
The charge agent may have been removed during the washing processes (solvent extraction) with acetone and methanol, since both solvents dissolved the charge agent arizona home loan purchase .
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