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Thanks to an unusually blue shifted Trp fluorescence spectrum of native azurin, a measure of the protein fraction remaining native after freezing-thawing can be obtained directly from the residual fluorescence intensity at 300 nm (λ^sub ex^ = 292 nm), a wavelength where denatured-aggregated proteins were found to contribute little to the overall intensity apply equity home loan online .
Also, LDH, which is notoriously unstable to freezing (14-16), was found to exhibit the largest binding capacity among the proteins examined in this study apply equity home loan online .
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Incubation of the heat-denatured samples at 24°C leads to gradual disk reconstitution accompanied by a reduction in the amount of lipid-poor apoA-2 ( Fig apply equity home loan online .
Chemical unfolding kinetics of the apoA-2:DMPC disks was probed in denaturant-jump experiments apply equity home loan online .
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The mechanism for doing so remains elusive, but will continue to be investigated apply equity home loan online .
Hence, a quasi-docked conformation could be visited many times during the course of hairpin ribozyme folding, thereby increasing the probability of forming the key tertiary contacts required for stable docking of loops A and B apply equity home loan online .
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