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. EI^sup sc^ was dephosphorylated by overnight dialysis at 278 K against phosphate potassium buffer (10 mM, pH 7
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. As a consequence the obtained transition temperatures are strongly depending on the heating rates (for a more extended discussion on this subject see (17))
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. Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state
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