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. DSC was used to investigate whether the thermal denaturation of ACYI was reversible or not
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. Biophysical Characterization of the Enzyme I of the Streptomyces coelicolor Phosphoenolpyruvate:Sugar Phosphotransferase SystemHurtado-Gómez, EstefaníaABSTRACT The first protein in the bacterial phosphoenolpyruvate (PEP):sugar phosphotransferase system is the homodimeric 60-kDa enzyme I (EI), which autophosphorylates in the presence of PEP and Mg^sup 2+^
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. In EI^sup sc^, although its sequence homology and its structure are similar to those of other EI proteins, the calorimetric equilibrium unfolding, as shown by DSC, is different
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. Structure and confomiational stability of the enzyme I of Slreplomyces coelicolor explored by KMR and circular dichroism
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. In the following, this sample is denoted as DmAChE-H2O
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. The fluorescence intensity of the different probes in the presence of the unfolded monomer state is shown in Fig
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. The final concentrations were 5 mM MgCl^sub 2^ and 2
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. These data suggest that the percentages of helical structure in EI^sup sc^ at the extremes of pH were increased
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