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14), also known as Acylase I (ACYI), is a homodimeric enzyme containing one zinc ion per subunit (4-6)
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Reversibility of the thermal transition was examined by reheating the samples after cooling from the first scan
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1, are turned off, we obtain Flory's freely rotating chain model (43), albeit with a constraint that the peptide units are all in a trans configuration
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Understanding protein folding via free energy surfaces from theory and experiment
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Here, p^sub A^ and p^sub B^ are the populations of the states A and B, Δ^sub ex^ = Ω^sub A^ - Ω^sub B^ is the chemical shift difference between the two states, k^sub ex^ = k^sub A[arrow right]B^ / p^sub B^ = k^sub B[arrow right]A^ / p^sub A^ is the rate constant for the exchange process, δ is the delay of the δ-180°-δ CPMG block and ω^sub I^ is the frequency of nucleus I
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That a two-contribution model is needed follows from a comparison of average experimental and theoretical R^sub 1^ values
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© 2005 by the Biophysical Society0006-3495/05/08/1214/13 $2
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Therefore, the widely accepted correlation between cation binding and compaction potential is not an absolute
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Fluorometric determination of amyloid fibrils in vitro using the fluorescent dye, Thioflavine T
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These enzymes deplete the polar HDL surface of the proteins or phospholipids and/or increase the apolar lipid core, thereby creating a mismatch between the particle surface and its core (clay et al
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Moreover, protein unfolding and dissociation in this transition lead to thermodynamically irreversible disk fusion, as indicated by the DSC, EM, and gel electrophoresis data (Fig
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van Gunsteren,[dagger] and Alexandre M
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