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. , 1997), suggesting a high degree of intersubunit cooperativity in the reactivation of the enzyme
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. Since no crystal structure of ACYI is available, all four of the components (A and S, I, II, and III) of Trp fluorescence defined previously (33,34) were included in the curve-fitting process
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. For each of the sequences shown in Table 1
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. Interpretations suggesting that highly denatured proteins might he embryos of their native-state counterparts must he treated with extreme caution because there is no sound theoretical basis for such proposals
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. coelicolor were obtained by homology modeling
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. It is known from various previous studies that for nearly all รก-amylases both thermal and GndHCl-induced unfolding appear as an irreversible process (17-19)
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. In the experiments with samples with aggregated proteins, the monomeric form was mixed with a small contribution from considerably larger particles (giving essentially a baseline), making a detailed analysis difficult
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. This supports the proposed mechanism of the early fibrils shown in Fig
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