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, hydrolyzed whey protein) with added vitamins, oligesaccharide, iron, docusahexaenoic acid (DHA) and lactoferrin
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1°C/min reveals for all protein concentrations λ^sub max^-values around 345 nm for the unfolded state
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The newly frozen samples were then allowed to anneal for 12 h at -5°C, followed by a final equilibration of 2 h at the temperature selected for fluorescence measurements (-5 and -13°C)
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For colloidal systems, the initial effects of attraction, particularly at high volume fractions, is a reduction in viscosity as the initial clustering of particles increases the free volume available for structural rearrangement (1)
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Macromolecular crowding: qualitative and semiquantitative successes, quantitative challenges
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This procedure also suggests a helpful way of conceptualizing links between the apparently divergent views of the folding funnel, collision-diffusion, nucleation-condensation and topomer search models
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Our results also highlight the ability of the diffusion spFRET method to resolve conformational subpopulations important during the course of RNA folding
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