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. The folding of ACYI has been characterized to be a three-state process involving an inactive dimeric intermediate with molten globule-like characteristics (18,19)
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. It was suggested, with the help of some computational studies, that this enhanced stability of the tetramer is mainly due to glycosylation and some stabilizing ionic interactions at the oligomeric interface (18)
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. The ANS binding test extended to other proteins confirmed a widespread stressed condition of the globular structure in ice, indicating that oligomers' proteins are more susceptible to unfolding than monomers', presumably because the macromolecule can be further destabilized by subunit dissociation
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. The observed differences in the lipid peak integrals in the cytoplasm also affirm earlier studies of yolk distribution within the oocyte (31) and agree well with triglycerides being the major neutral lipid component of many types of cells (33)
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. MATERIALS AND METHODSMaterialsBLG was obtained from Sigma-Aldrich (Poole, UK) (L0130) and is a mixture of genetic variants A and B
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. There are four families of Gα-subunits, and phospholipase C-β(PLCβ) is the main effector of the Gα^sub q^ family (Rebecchi and Pentyala, 2000; Rhee, 2001)
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. Pseudopercis semifasciata is a multiple spawner with low batch fecundity and an extended reproductive season (Macchi et al
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. Evidence for rapid interconversion of extended and quasi-docked ribozyme conformationsA notable feature of the SL 4WJ in the presence of high Mg^sup 2+^ concentrations is the markedly asymmetric peak shape in the FRET efficiency histogram, with pronounced tailing on the low-E side of the peak (Fig
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