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. (Rudy had cynically labeled his own memo "Murder, Inc")
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. Five-hundred milligrams of holomyoglobin (purchased from Sigma, St
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. Protein concentration was 4 µM, unless otherwise indicated
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. 7 K when both effectors were present)
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. The distortion of the native structure inferred from ANS binding was found to draw a parallel with the extent of irreversible denaturation by freeze-thawing, suggesting that these altered conformations play a direct role on freeze damage
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. Rapid formation of IMCs inhibited the wettability of Sn-2
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. This new type of CaM interaction is the first example where two interacting CaM molecules interact with a dimeric target
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. The upper platen was of ceramic (diameter, 25 mm or 50 mm)
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. 4, we show that Gα^sub s^ is destabilized under pressure even at relatively high lipid concentrations, although we note that membrane binding of Gα^sub s^ is weaker than Gβγ (i
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. , 2002) indicate similar rank order for the DMPC disk stability
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. Furthermore, information on the effect on folding rate of replacing specific residues via mutation or "Φ-value" analysis (Fersht, 2000) needs to be taken together with correlations of contact order
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. PFROMMDepartment of Chemical Engineering, Kansas State University, Manhattan, Kansas 66506, U
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. Dihydrocytochalasin B enhances transformining growth factor-beta-induced reexpression of the differentiated chondrocyte phenotype without stimulation of collagen synthesis
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. Reno: University of Nevada Press
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. The former corresponds to that of the intermediate, whereas the latter peak represents the partially denatured population
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. In fact, defective efferocytosis has been demonstrated in patients with rheumatoid arthritis, systemic lupus erythematosus, glomerulonephritis, and atherosclerosis
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. Thus, weakening the azurin fold by the mutations leads to the creation of additional binding sites characterized by a broad distribution of binding affinities, a large fraction of which are of stronger affinity than in the WT protein
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.586 µm for Sn-Pb and 0
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. In the same study we could also conclude that SEF2-1mp lacks any well-defined secondary structure when interacting with CaM, an observation very unusual for CaM-bound peptides
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. Liquid-liquid phase separation was observed at pH 5
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.Very little is known about the ecology and behavior of P
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. 5), the entropy buildup curves show a substantial jump at the appending point when the trajectory of the free form is appended to that of the complex form (dashed line), indicating that the free molecule samples conformational space not visited in the complex simulation (case A; Fig
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. Sometimes a mild cooked flavor is developed deliberately to impart a readily distinguishable flavor to the ice cream
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.In the first application of diffusion spFRET to study RNA folding, we have investigated the magnesium-induced tertiary folding of the hairpin ribozyme
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. At low Mg^sup 2+^, all three ribozymes show a single peak at low E, reflecting an extended, undocked state
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. In contrast, our results imply the presence of a folding intermediate with the two arms in close enough proximity to allow formation of specific loop-loop contacts
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