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The thermal stability of EI^sup SC^, in the absence of PEP and Mg^sup 2+^, was maximal at pH 7
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EIN is composed of an HPr-binding α-helical subdomain and an α/β subdomain containing the active site histidine
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This modeled structure has allowed us to explain the experimental conformational and thennodynamic features of the protein
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Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added
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Such a correlation diagram of R^sub ex^ and ΔR^sub ex^ is shown in Fig
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), Magnus Bergwall's Foundation (P
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In apoA-2:DMPC complexes, the repeatability of the high-temperature transition that involves unfolding of lipidated apoA-2 (Fig
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The distributional ellipses calculated for summer and fall from the positive stations were small and widely separated
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In other words, the second MD trajectory segment covers a small subspace of the conformational space sampled by the first segment and hence reduces the overall size of the elements of the covariance matrix σ through averaging over the combined trajectories
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To do so, we estimated the impact of Type 1 and Type 2 RP on [PriceURNSD
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0013,7228,085Cumulative effect post-RP 33,454 <
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. The changes of thermodynamic parameters (enthalpy change ((Delta)H), entropy change ((Delta)S) and heat capacity change ((delta)C^sub p^)) corresponding to the transitions from N to MG (molten globule state) or MG to U (unfolded state), were summarized in the previous report (Nishii et al
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